atSpiro
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Smart laboratory device helps biotech/pharmaceutical companies develop microorganism for production.
23/04/2025
𝗟𝗮𝗰𝗸 𝗼𝗳 𝗽𝗛 𝗰𝗼𝗻𝘁𝗿𝗼𝗹 𝗯𝗼𝘁𝘁𝗹𝗲𝗻𝗲𝗰𝗸𝘀 𝘆𝗼𝘂𝗿 𝗰𝗲𝗹𝗹 𝗲𝘅𝗽𝗮𝗻𝘀𝗶𝗼𝗻.
In shake flask cultures pH control is often overlooked – but it matters.
Together with Uncommon Bio, we tested porcine iPSC expansion with and without automated pH regulation in a standard shake flask format.
📈 𝗧𝗵𝗲 𝗿𝗲𝘀𝘂𝗹𝘁: 55% increase in viable cell yield with pH control using the ShakeReactor.
🧪 Same medium, same conditions — the only change was automated pH maintenance (7.2–7.4).
📸 Side-by-side image: iPSC aggregates under 4x magnification:
𝗟𝗲𝗳𝘁: standard protocol without pH control
𝗥𝗶𝗴𝗵𝘁: same protocol with automated pH control using the ShakeReactor
This approach doesn't just improve growth — it helps bridge the gap between R&D and scalable production, by allowing you to simulate production like bioreactor conditions earlier in R&D.
📥 Download the white paper to learn more: https://shorturl.at/oQt8a
23/04/2025
Scaling up? Oxygen transfer could be your biggest blind spot.
Shake flasks are a convenient tool for optimizing media, temperature, and pH. But when it’s time to scale up to bioreactors, things often don’t go as planned- leading to costly delays, unpredictable results, and wasted time.
Oxygen limitation is often to blame. Ensuring sufficient oxygenation starts with characterizing the oxygen mass transfer coefficient, kLa, for your conditions. Historically, it was difficult to measure in shake flasks due to the lack of dissolved oxygen monitoring. But new technology has changed that.
The ShakeReactor adds continuous monitoring of dissolved oxygen, making it simple to measure to characterize kLa in shake flasks.
📈 Curious about kLa values for standard conditions or want to learn how to characterize it in your setup?
📥Download the whitepaper: https://shorturl.at/HE7QX
15/08/2024
We don’t like to brag but look at the wide range of organisms our customers have successfully cultured in the ShakeReactor!
The ShakeReactor is an easy and versatile bioreactor designed to streamline lab-scale cultivations, whether you're working with bacteria, yeast, fungi, algae or mammalian cells. By adding DO and pH measurement and automated feeding – controlled by a user friendly software, the ShakeReactor delivers enhanced control and better performance compared to flask cultivations.
Ready to optimize your lab's workflow? Contact us at atSpiro.com to learn more about how the ShakeReactor can enhance your research!
Credits to picture: https://t.ly/p72ha
🔬 E. coli in minimal media with maximum monitoring! 🔍✨
See when your organisms grow, lack oxygen, or run out of nutrients by monitoring dissolved oxygen in your shake flasks using the ShakeReactor.
The following video shows E. coli in buffered minimal media:
📈 Exponential growth is clearly observed in the sharp drop in dissolved oxygen, perfectly aligning with the opacity change of the media.
💡 0% dissolved oxygen readings during exponential growth indicate insufficient shaking speed or inadequate fill volume.
👩🔬 pH fluctuates even in buffered media, showing the need for a pH control.
Monitoring alone allows you to gain valuable insights from your cultivations, but in addition to that, the ShakeReactor can also accurately control your experiments using its three programmable pumps.
Know what your cells are up to! Visit atspiro.com/demo to book a trial, or write us on [email protected]
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Internet side
Adresse
Maskinvej 5, C/O Alfa Laval Copenhagen A/S
Søborg
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