Life Extension Beyond Borders Association

This study develops an immune cell atlas of “Longevity Molecular Tag” through the integration of single-cell and bulk transcriptomic data. The findings indicate that centenarians attain an immune equilibrium conducive to healthy longevity via the remodeling of cytotoxic immune cell lineages and the fine-tuning of inflammatory responses, rather than through generalized immunosuppression. Our integrative Scissor framework, which couples lifespan single-cell transcriptomes with bulk transcriptomic profiles from Guangxi centenarians, identifies longevity-associated Scissor+ subpopulations predominantly within NK cells, CD8+ T cells, γδ T cells, and megakaryocytes, and longevity-negative Scissor− subpopulations mainly within CD4+ T cells, B cells, DCs, and erythrocytes; it further prioritizes genetically supported candidate axes based on eQTL–GWAS colocalization (rs3793537–GLIPR2/CD72/TLN1 and rs8019902–TRDV2/TRDC) as putative molecular targets linked to extended lifespan, thereby offering novel avenues for interventions aimed at fostering healthy aging.

The Immune Cell Atlas of “Longevity Molecular Tag”: Identification of Principal Immune Cell Subsets and Their Underlying Molecular Regulatory Mechanisms This study aims to develop an immune cell atlas of the “Longevity Molecular Tags” by employing the Scissor algorithm to identify key immune cell subsets associated with longevity phenotypes. Subseque...