Sapidyne Instruments Inc.

"In an effort to provide faster, economical and reliable PAH analyses, a variety of immunoassay methods have been examined for the detection and quantification of PAH in environmental samples (EPA, 1996a, EPA, 1996b, Spier et al., 2012, Behera et al., 2018). Our lab has developed a rapid, near-real-time method for PAH quantitation using the KinExA Inline Biosensor (Sapidyne Instruments, Boise, ID) and a mouse-derived anti-pyrene-butyric acid monoclonal antibody, 2G8, with previously demonstrated uniform selectivity for a range of 3–5 ring PAHs (Li et al., 2016). Additionally, the biosensor is a user-friendly instrument that can directly analyze environmental samples (i.e requires minimal sample preparation). It has been used to quantify PAH in porewater samples in the Chesapeake Bay and Houston ship channel and has demonstrated strong positive correlation with PAH measurements of sediment porewater when using passive sampling and GC–MS (Hartzell et al., 2017, Conder et al., 2021, Camargo et al., 2022)."

A novel antibody-based biosensor method for the rapid measurement of PAH contamination in oysters Conventional PAH analytical methods are time-consuming and expensive, limiting their utility in time sensitive events (i.e. oil spills and floods) or …

"Therefore, competition between the solution complex and the solid phase is “kinetically excluded” and the solution equilibrium is not altered during KinExA experiments. The signals generated from the captured CD40 and/or integrins, which are directly proportional to the concentration of free integrin in the equilibrated samples, are used to generate a binding curve, measured in a series. The KinExA method is a means to obtain true solution phase measurements."

Modulating CD40 and integrin signaling in the proinflammatory nexus using a 15-amino acid peptide, KGYY15. CD40-signaling has long been a target in autoimmunity. Attempts to block signaling between CD40 and CD154 during clinical trials using monoclonal anti…

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